1ME5
Crystal Structure of Mycobacterium Tuberculosis Alkylperoxidase AhpD H132Q Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.119, 58.652, 88.914 |
| Unit cell angles | 90.00, 120.47, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.2349 |
| Rwork | 0.223 |
| R-free | 0.35040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lw1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.387 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 16445 | |
| Completeness [%] | 93.6 | 87.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | AMMONIUM ACETATE, SODIUM CITRATE, PEG 4000, MOPS, KCL, EDTA, GLYCEROL, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
