1MDZ

Crystal structure of ArnB aminotransferase with cycloserine and pyridoxal 5' phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 43 21 2
Unit cell lengths	90.904, 90.904, 127.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 2.080 *
R-factor	0.223 *
Rwork	0.212
R-free	0.25000
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011 *
RMSD bond angle	2.020 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600 *	2.180
High resolution limit [Å]	2.080 *	2.070
Rmerge	0.200 *	1.419 *
Total number of observations	440660 *
Number of reflections	33311
Completeness [%]	99.4 *	96.3 *
Redundancy	13.2 *	8.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	20 *	sodium citrate, PEG 10000, beta-mercaptoethanol, pH 5.5, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)	pH5.5
3	1	reservoir	PEG10000	18-22 (%)
4	1	reservoir	beta-mercaptoethanol	1 (mM)