1MDC

CRYSTALLIZATION, STRUCTURE DETERMINATION AND LEAST-SQUARES REFINEMENT TO 1.75 ANGSTROMS RESOLUTION OF THE FATTY-ACID-BINDING PROTEIN ISOLATED FROM MANDUCA SEXTA L

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	27.500, 70.800, 28.500
Unit cell angles	90.00, 90.50, 90.00

Refinement procedure

Resolution	30.000 - 1.750
R-factor	0.173
RMSD bond length	0.016
RMSD bond angle	2.680
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.750 *
Total number of observations	37749 *
Number of reflections	10674 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5 *		macro-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)	0.02 ml
2	1	drop	HEPES	10 (mM)	pH7.0
3	1	drop	ammonium sulfate	1.4 (M)	0.02 ml
4	1	drop	Na+-K+ succinate	50 (mM)
5	1	drop		5 (mM)
6	1	reservoir	ammonium sulfate	1.4 (M)
7	1	reservoir	succinate	50 (mM)