1MD0
CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5415 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.350, 66.670, 83.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.000 |
| Rwork | 0.209 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k79 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.176 |
| Number of reflections | 19649 | |
| <I/σ(I)> | 21.4 | 4.9 |
| Completeness [%] | 94.4 | 68.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | 50mM Sodium Citrate, 20% Peg4000, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.27 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 4 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 5 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 6 | 1 | reservoir | PEG4000 | 20 (%) |
