1MCY

SPERM WHALE MYOGLOBIN (MUTANT WITH INITIATOR MET AND WITH HIS 64 REPLACED BY GLN, LEU 29 REPLACED BY PHE

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 6
Unit cell lengths	91.390, 91.390, 45.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 1.700
R-factor	0.182
Rwork	0.182
RMSD bond length	0.020
RMSD bond angle	1.820
Data reduction software	RIGAKU
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.700
Rmerge	0.109
Total number of observations	151315 *
Number of reflections	25787
Completeness [%]	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	70 (mg/ml)
2	1	1	ammonium sulfate	2.2-2.6 (M)
3	1	1	Tris-HCl	20 (mM)