1MCX
STRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 129.483, 129.483, 66.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.030 |
| Rwork | 0.196 |
| R-free | 0.23110 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hm6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.061 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | |
| High resolution limit [Å] | 2.030 | 2.030 * |
| Rmerge | 0.117 | 0.891 * |
| Total number of observations | 568454 * | |
| Number of reflections | 37069 | |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 97.9 * | 95.7 * |
| Redundancy | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 277 | PEG 1000, imidazol, calciumacetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG1000 | 10 (%(w/v)) | |
| 2 | 1 | 1 | imidazole | 50 (mM) | pH8.0 |
| 3 | 1 | 1 | cadmium acetate | 100 (mM) |
