1MCX

STRUCTURE OF FULL-LENGTH ANNEXIN A1 IN THE PRESENCE OF CALCIUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	129.483, 129.483, 66.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.030
Rwork	0.196
R-free	0.23110 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hm6
RMSD bond length	0.005
RMSD bond angle	1.061 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000
High resolution limit [Å]	2.030	2.030 *
Rmerge	0.117	0.891 *
Total number of observations	568454 *
Number of reflections	37069
<I/σ(I)>	27.2
Completeness [%]	97.9 *	95.7 *
Redundancy	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8	277	PEG 1000, imidazol, calciumacetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG1000	10 (%(w/v))
2	1	1	imidazole	50 (mM)	pH8.0
3	1	1	cadmium acetate	100 (mM)