1MCT

THE REFINED 1.6 ANGSTROMS RESOLUTION CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN PORCINE BETA-TRYPSIN AND MCTI-A, A TRYPSIN INHIBITOR OF SQUASH FAMILY

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	62.650, 62.650, 124.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 * - 1.600
R-factor	0.167
Rwork	0.167
RMSD bond length	0.011
RMSD bond angle	2.200
Phasing software	SQUASH
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.083 *
Total number of observations	264792 *
Number of reflections	32238 *
Completeness [%]	83.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	0.2 (M)
2	1	drop		35 (%sat)
3	1	reservoir	sodium phosphate	0.2 (M)
4	1	reservoir		85 (%sat)