1MC0

Regulatory Segment of Mouse 3',5'-Cyclic Nucleotide Phosphodiesterase 2A, Containing the GAF A and GAF B Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0332
Spacegroup name	F 2 2 2
Unit cell lengths	134.200, 136.200, 149.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.860
R-factor	0.221
Rwork	0.221
R-free	0.26900 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	21.600 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	2.970
High resolution limit [Å]	2.860	2.860
Rmerge	0.058 *	0.408 *
Number of reflections	15282
Completeness [%]	95.9	87.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	294	1,4 butanediol, sodium acetate, dithiothreitol, EDTA, Tris, sodium chloride, 3',5' guanosine monophosphate, leupeptin, pepstatin, phenylmethylsulfonyl flouride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2-5 (mg/ml)
10	1	reservoir	1,4butanediol	16-26 (%)
11	1	reservoir	sodium acetate	100 (mM)	pH5.0
12	1	reservoir	beta-mercaptoethanol	140 (mM)
2	1	drop		20 (mM)
3	1	drop	Tris	10 (mM)	pH7.5
4	1	drop	dithiothreitol	10 (mM)
5	1	drop	EDTA	0.1 (mM)
6	1	drop	PMSF	0.1 (mM)
7	1	drop	leupeptin	0.001 (mg/ml)
8	1	drop	pepstain A	0.001 (mg/ml)
9	1	drop	Na 3',5'cGMP	2 (mM)