1MBZ
BETA-LACTAM SYNTHETASE WITH TRAPPED INTERMEDIATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.390, 97.154, 81.087 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 29.000 * - 2.470 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jgt |
| RMSD bond length | 0.028 |
| RMSD bond angle | 24.000 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 * | 2.550 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.094 * | 0.292 * |
| Total number of observations | 382028 * | |
| Number of reflections | 34018 * | |
| Completeness [%] | 99.1 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 296 | Miller, M.T., (2001) Nature Struct. Biol., 8, 684. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG4000 | 7 (%(w/v)) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | glycerol | 7 (%(v/v)) | |
| 6 | 1 | reservoir | Tris | 80 (mM) | pH8.0 |
