1MBY
Murine Sak Polo Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2000-12-13 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9788,0.9790,0.9637,0.9945 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 51.782, 51.782, 146.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.231 |
| R-free | 0.24750 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.510 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Jeffamine M-600, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH8.0 |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |
| 5 | 1 | reservoir | Jeffamine M-600 | 32.5 (%(v/v)) | |
| 6 | 1 | reservoir | 200 (mM) |
