1MBX

CRYSTAL STRUCTURE ANALYSIS OF ClpSN WITH TRANSITION METAL ION BOUND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-07-26
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	87.436, 87.436, 212.955
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.300 *
Rwork	0.208
R-free	0.23600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mbu
RMSD bond length	0.012 *
RMSD bond angle	1.460 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.250 *	2.200
Rmerge	0.045 *	0.638 *
Number of reflections	45812 *
<I/σ(I)>	46.1	1.4
Completeness [%]	95.9 *	69.9 *
Redundancy	7.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	294	glycerol, bis-tris , yttrium chloride, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 21K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bis-Tris	0.1 (M)	pH6.5
2	1	reservoir	glycerol	32 (%)
3	1	reservoir	yttrium chloride	10-15 (mM)
4	1	drop	protein	10 (mg/ml)