1MBL

A catalytically-impaired class A beta-lactamase: 2 Angstroms crystal structure and kinetics of the Bacillus licheniformis E166A mutant

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	67.200, 94.100, 43.800
Unit cell angles	90.00, 104.50, 90.00

Refinement procedure

Resolution	10.000 * - 2.000
R-factor	0.172 *
RMSD bond length	0.005
RMSD bond angle	1.500
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.051 *
Total number of observations	87548 *
Number of reflections	29095 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	2.5 (mg/ml)
2	1	drop	penicillin G	5 (mM)
3	1	drop		2 (mM)
4	1	drop		2 (mM)
5	1	reservoir	methoxypolyethyleneglycol	25 (%)
6	1	reservoir	sodium cacodylate-HEPES	25 (mM)