1MAY
BETA-TRYPSIN PHOSPHONATE INHIBITED
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-01-16 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.950, 58.550, 67.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.680 |
| Data reduction software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Rmerge | 0.120 |
| Number of reflections | 28987 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | beta-trypsin | 10-15 (mg/ml) | |
| 2 | 1 | drop | MES | 5.0 (mM) | |
| 3 | 1 | drop | 1.0 (mM) | ||
| 4 | 1 | drop | benzamidine | 2.5 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 0.9 (M) | |
| 6 | 1 | reservoir | MES | 5.0 (mM) | |
| 7 | 1 | reservoir | 1.0 (mM) | ||
| 8 | 1 | reservoir | benzamidine | 2.5 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 1.8 (M) |
