1MAP

CRYSTAL STRUCTURES OF TRUE ENZYMATIC REACTION INTERMEDIATES: ASPARTATE AND GLUTAMATE KETIMINES IN ASPARTATE AMINOTRANSFERASE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	70.200, 91.600, 129.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.169
RMSD bond length	0.009
RMSD bond angle	23.900 *
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.400 *
Rmerge	0.089 *
Total number of observations	43358 *
Number of reflections	15053 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	19-22 (%(w/v))
2	1	reservoir	maleate