1MAE

The Active Site Structure of Methylamine Dehydrogenase: Hydrazines Identify C6 as the Reactive Site of the Tryptophan Derived Quinone Cofactor

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	129.784, 129.784, 104.334
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.800
R-factor	0.183
RMSD bond length	0.018
RMSD bond angle	2.800
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.800 *
Rmerge	0.073 *	0.157 *
Total number of observations	125106 *
Number of reflections	25377 *
Completeness [%]	95.7 *	92.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	4 *	taken from Ubbink, M. et al (1991). Eur. J. Biochem., 202, 1003-1012. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)
3	1	reservoir	ammonium sulfate	37-42 (%sat)
4	1	reservoir	sodium acetate	0.1 (M)