1MAB

RAT LIVER F1-ATPASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MACSCIENCE
Spacegroup name	H 3 2
Unit cell lengths	143.670, 143.670, 361.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.217
Rwork	0.217
R-free	0.29000
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	1.730 *
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.800
Rmerge	0.103 *
Number of reflections	35334
<I/σ(I)>	7.1
Completeness [%]	85.3
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.45 *		Amzel, L.M., (1978) J.Biol.Chem., 253, 2067. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	potassium phosphate	200 (mM)
2	1	1	ATP	5 (mM)
3	1	1	protein	15 (mg/ml)
4	1	2	potassium phosphate	200 (mM)
5	1	2	ATP	5 (mM)
6	1	2	ammonium sulfate	1 (M)