1MAB
RAT LIVER F1-ATPASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 143.670, 143.670, 361.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.29000 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.730 * |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.103 * |
| Number of reflections | 35334 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 85.3 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.45 * | Amzel, L.M., (1978) J.Biol.Chem., 253, 2067. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | potassium phosphate | 200 (mM) | |
| 2 | 1 | 1 | ATP | 5 (mM) | |
| 3 | 1 | 1 | protein | 15 (mg/ml) | |
| 4 | 1 | 2 | potassium phosphate | 200 (mM) | |
| 5 | 1 | 2 | ATP | 5 (mM) | |
| 6 | 1 | 2 | ammonium sulfate | 1 (M) |
