1M9M
human endothelial nitric oxide synthase with 6-nitroindazole bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.757, 90.782, 155.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.960 |
| Rwork | 0.193 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.046 | 0.321 |
| Total number of observations | 290276 * | |
| Number of reflections | 69487 | 4142 * |
| <I/σ(I)> | 28.4 | 2.7 |
| Completeness [%] | 96.6 | 87.2 |
| Redundancy | 4.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 298 | NaCl, MPD, PEG, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 25 (mM) | pH8.0 |
| 3 | 1 | drop | 400 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 4 (mM) | |
| 5 | 1 | drop | BH4 | 0.010 (mM) | |
| 6 | 1 | reservoir | 200-400 (mM) | ||
| 7 | 1 | reservoir | MPD | 13 (%) | or 2-propamol |
| 8 | 1 | reservoir | PEG3350 | 2-5 (%) |
