1M8W
CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-19 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0093 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 262.730, 37.730, 82.520 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 42.680 - 2.200 |
| R-factor | 0.225 * |
| Rwork | 0.224 |
| R-free | 0.29000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IB3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.060 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.680 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.042 * | 0.359 * |
| Number of reflections | 40790 | 3936 * |
| <I/σ(I)> | 25.9 | 3 |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 20 * | PEG 3350, lithium sulfate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.5 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 14 (%(w/v)) | |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
