1M8A
Human MIP-3alpha/CCL20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 70.822, 70.822, 71.768 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.1931 |
| Rwork | 0.190 |
| R-free | 0.23800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eqt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.623 |
| Total number of observations | 99101 * | |
| Number of reflections | 21953 | |
| <I/σ(I)> | 23.8 | 1.9 |
| Completeness [%] | 97.5 | 96.8 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 12 * | isopropanol, sodium citrate, N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | in water |
| 2 | 1 | reservoir | isopropanol | 20 (%) | |
| 3 | 1 | reservoir | trisodium citrate | 200 (mM) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
