1M7Y
Crystal structure of apple ACC synthase in complex with L-aminoethoxyvinylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.79955 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.310, 61.140, 77.140 |
| Unit cell angles | 90.00, 123.36, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.600 |
| Rwork | 0.205 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.052 | 0.281 |
| Number of reflections | 50540 * | |
| <I/σ(I)> | 16.5 | 2.9 |
| Completeness [%] | 95.2 | 76 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 * | 293 | MPD, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | pH7.9 |
| 3 | 1 | drop | PLP | 10000 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | AVG | 4 (mM) | |
| 6 | 1 | reservoir | MPD | 30 (%(v/v)) | |
| 7 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
