1M7U

Crystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	108
Detector technology	CCD
Collection date	2001-06-29
Detector	BRANDEIS - B4
Wavelength(s)	1.10
Spacegroup name	P 31 2 1
Unit cell lengths	63.960, 63.960, 285.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.232
R-free	0.29000
Structure solution method	SIRAS
RMSD bond length	0.008 *
RMSD bond angle	1.430 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.860
High resolution limit [Å]	2.800	2.800
Rmerge	0.067 *	0.209 *
Number of reflections	17150
<I/σ(I)>	18.6	6.8
Completeness [%]	98.7 *	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294 *	ammonium sulfate, citric acid, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.6 (M)
2	1	drop	sodium citrate	0.1 (M)	pH5.6
3	1	drop	HEPES	50 (mM)	pH7.0
4	1	drop		0.2 (M)
5	1	reservoir	ammonium sulfate	1.6 (M)	pH5.6
6	1	reservoir	sodium citrate