1M7S

Crystal Structure Analysis of Catalase CatF of Pseudomonas syringae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-21
Detector	MARRESEARCH
Wavelength(s)	0.93300
Spacegroup name	P 1 21 1
Unit cell lengths	60.630, 153.870, 109.190
Unit cell angles	90.00, 102.80, 90.00

Refinement procedure

Resolution	21.900 - 1.800
R-factor	0.183
Rwork	0.187
R-free	0.24100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011 *
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.900 *	1.870
High resolution limit [Å]	1.800	1.800
Rmerge	0.084 *	0.210
Number of reflections	175279	17810 *
<I/σ(I)>		5.9
Completeness [%]	97.8	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 4000, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	PEG4000	12 (%)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.