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1M7P

Plasmodium Falciparum Triosephosphate isomerase (PfTIM) compled to substrate analog glycerol-3-phosphate (G3P).

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]295
Detector technologyIMAGE PLATE
Collection date1999-06-11
DetectorMARRESEARCH
Wavelength(s)1.5418
Spacegroup nameP 1 21 1
Unit cell lengths54.124, 51.082, 89.549
Unit cell angles90.00, 91.39, 90.00
Refinement procedure
Resolution20.000 - 2.400
Rwork0.179
R-free0.21900

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)Dimer of Unbound PfTIM; PDB code 1YDV
RMSD bond length0.008
RMSD bond angle1.486

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (0.4)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.490
High resolution limit [Å]2.4002.400
Rmerge0.115

*

0.356

*

Total number of observations98166

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Number of reflections19337
<I/σ(I)>12.34.1
Completeness [%]96.596.4
Redundancy5.14.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5

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29512% to 20% PEG 1450 in 100mm Sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP at 295K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirPEG14508-24 (%)
21reservoirsodium acetate100 (mM)pH7.5
31dropprotein10 (mg/ml)

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PDB entries from 2024-12-25

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