1M7G
Crystal structure of APS kinase from Penicillium Chrysogenum: Ternary structure with ADP and APS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.520, 84.320, 137.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.430 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.049 | 0.399 |
| Total number of observations | 3135818 * | |
| Number of reflections | 174517 * | |
| <I/σ(I)> | 13.2 | 4.5 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 17.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Na monobasic phosphate, K dibasic phosphate, succinate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | drop | MgADP | 3 (mM) | |
| 4 | 1 | drop | APS | 3 (mM) | |
| 5 | 1 | reservoir | 1.7 (M) | ||
| 6 | 1 | reservoir | 0.3 (M) | ||
| 7 | 1 | reservoir | sodium succinate | 0.1 (M) | pH4.0 |
