1M6U

Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	2001-05-16
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	101.275, 64.897, 92.419
Unit cell angles	90.00, 107.52, 90.00

Refinement procedure

Resolution	19.960 - 2.300
Rwork	0.226
R-free	0.27100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ndt80 pdb entry 1M7U
RMSD bond length	0.006 *
RMSD bond angle	1.370 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.084 *	0.241 *
Number of reflections	25573
<I/σ(I)>	15.8	3.3
Completeness [%]	95.7	84.9
Redundancy	5.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	Montano, S.P., (2002) Acta Crystallogr., D58, 2127. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.9 (mM)
2	1	reservoir	lithium sulfate	1.85-1.9 (M)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.6