1M6R
Crystal structure of rGd(CGCGCG) forming hexamer Z-DNA duplex with 5'-(rG) overhang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-08 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.960, 31.470, 44.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.540 |
| Rwork | 0.162 |
| R-free | 0.23500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.650 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.049 | 0.195 |
| Total number of observations | 12118 * | |
| Number of reflections | 3576 | |
| Completeness [%] | 87.1 | 79.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2-propanol (at reservoir), Spermine tetrachloride, magnesium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Spermine tetrachloride | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 2 | 2-propanol | ||
| 5 | 1 | 2 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | chimera | 1 (mM) | single strand concentration |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | pH6.5 |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | spermine tetrachloride | 1 (mM) | |
| 5 | 1 | reservoir | 2-propanol | 10 (%) |
