1M6J
CRYSTAL STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ENTAMOEBA HISTOLYTICA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.782 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.729, 119.947, 50.392 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 1.500 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.20700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tim |
RMSD bond length | 0.004 |
RMSD bond angle | 21.500 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.750 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 * | |
Total number of observations | 360647 * | |
Number of reflections | 80420 * | |
<I/σ(I)> | 15.7 | |
Completeness [%] | 99.4 * | 76.1 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | 28% PEG 1500, 30% 1,6-hexanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 291K, temperature 291.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG1500 | 28 (%(w/v)) | |
3 | 1 | reservoir | 1,6-hexanediol | 30 (%(v/v)) |