1M68
YCDX PROTEIN, TRINUCLEAR ZINC SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 77.350, 77.350, 80.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.16505 |
| Rwork | 0.162 |
| R-free | 0.22500 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1m65 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.041 | 0.102 |
| Number of reflections | 12596 | |
| <I/σ(I)> | 33.7 | 12.8 |
| Completeness [%] | 99.2 | 89.8 |
| Redundancy | 8.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris, 60% AS, 5 mM Zinc Acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 2 | 1 | reservoir | ammonium sulfate | 60 (%) | |
| 3 | 1 | reservoir | MPD | 3 (%) |
