1M5X
Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2001-03-05 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.510, 42.210, 71.320 |
| Unit cell angles | 73.34, 73.17, 71.09 |
Refinement procedure
| Resolution | 50.000 * - 2.250 |
| Rwork | 0.219 |
| R-free | 0.25300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | poly ananine I-CreI/DNA complex |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.030 * | 0.076 |
| Total number of observations | 38547 * | |
| Number of reflections | 19849 * | |
| <I/σ(I)> | 26.1 | 9.79 |
| Completeness [%] | 97.5 * | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | PEG 400, sodium chloride, calcium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 2 | NaCl | ||
| 5 | 1 | 2 | CaCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 19-22 (%) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH7.3-7.9 |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | 20 (mM) | ||
| 5 | 1 | drop | protein | 3.5 (mg/ml) |
