1M5H
Formylmethanofuran:tetrahydromethanopterin formyltransferase from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-06-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8452 |
Spacegroup name | P 1 |
Unit cell lengths | 77.100, 81.810, 99.100 |
Unit cell angles | 90.10, 110.04, 93.75 |
Refinement procedure
Resolution | 29.250 - 2.000 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Formylmethanofuran:Tetrahydromethanopterin Formyltransferase from Methanosarcina barkeri |
RMSD bond length | 0.006 |
RMSD bond angle | 24.200 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 * | 0.223 * |
Total number of observations | 839340 * | |
Number of reflections | 150792 | |
Completeness [%] | 86.0 | 67 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | Imidazol/Malate, KSCN, PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | MOPS | 10 (mM) | pH7.0 |
3 | 1 | reservoir | imidazole-malate | 0.1 (M) | |
4 | 1 | reservoir | KSCN | 0.2 (M) | |
5 | 1 | reservoir | PEG3350 | 23 (%) |