1M59
Crystal Structure of P40V Mutant of Trypsin-solubilized Fragment of Cytochrome b5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.709, 40.388, 39.299 |
| Unit cell angles | 90.00, 111.72, 90.00 |
Refinement procedure
| Resolution | 20.880 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1m20 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.900 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 7747 | |
| <I/σ(I)> | 21.1 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | phosphate buffer, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
