1M57
Structure of cytochrome c oxidase from Rhodobacter sphaeroides (EQ(I-286) mutant))
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 340.720, 340.720, 89.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 3.000 |
| R-factor | 0.293 |
| Rwork | 0.293 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.047 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.124 * | 0.410 * |
| Total number of observations | 163199 * | |
| Number of reflections | 71181 * | 6553 * |
| Completeness [%] | 89.9 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG400, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 10 | 1 | reservoir | 20 (mM) | ||
| 11 | 1 | reservoir | dodecylmaltoside | 0.014 (%) | |
| 2 | 1 | drop | decyl maltoside | 0.24 (%) | |
| 3 | 1 | drop | protein | 10-20 (mg/ml) | |
| 4 | 1 | reservoir | PEG400 | 18-22 (%) | |
| 5 | 1 | reservoir | 50-150 (mM) | ||
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
| 7 | 1 | reservoir | Tris | 10 (mM) | |
| 8 | 1 | reservoir | decylmaltoside | 0.24 (%) | |
| 9 | 1 | reservoir | heptanetriol | 2.5-3 (%) |
