1M55
Catalytic domain of the Adeno Associated Virus type 5 Rep protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-12-06 |
Detector | CUSTOM-MADE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.888, 73.455, 65.560 |
Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.400 |
Rwork | 0.178 |
R-free | 0.19600 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.360 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.430 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.062 | 0.404 |
Total number of observations | 373647 * | |
Number of reflections | 79390 | |
<I/σ(I)> | 10 | 2.68 |
Completeness [%] | 99.6 | 95.9 |
Redundancy | 4.71 | 2.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | PEG 8000, PEG 400, ZINC ACETATE, SODIUM CACODYLATE, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG400 | 10 (%(v/v)) | |
2 | 1 | drop | sodium cacodylate | 80 (mM) | pH6.5 |
3 | 1 | drop | zinc acetate | 24 (mM) | |
4 | 1 | drop | PEG8000 | 10 (%(v/v)) | |
5 | 1 | reservoir | cacodylate | 100 (mM) | |
6 | 1 | reservoir | zinc acetate | 30 (mM) | |
7 | 1 | reservoir | PEG8000 | 12.5 (%) |