1M4W

Thermophilic b-1,4-xylanase from Nonomuraea flexuosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-10-20
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	37.034, 37.034, 191.808
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.000 - 2.100
Rwork	0.146
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1enx
RMSD bond length	0.006 *
RMSD bond angle	1.439
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.107 *	0.293 *
Total number of observations	48587 *
Number of reflections	8541
<I/σ(I)>	17.9
Completeness [%]	97.6	92.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.8 M Ammonium sulphate, 0.1 M Sodium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	reservoir	ammonium sulfate	0.4 (M)
3	1	reservoir	sodium acetate	0.05 (M)	pH6.0