1M4R
CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.430, 61.610, 73.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.700 * - 2.000 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22000 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 * |
RMSD bond angle | 19.100 * |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.700 * | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.350 |
Total number of observations | 61846 * | |
Number of reflections | 16382 * | |
<I/σ(I)> | 14.5 | 3.8 |
Completeness [%] | 92.7 * | 82.3 |
Redundancy | 3.8 * | 3.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 300 | 0.9 sodium tartrate, TRITON X-100 detergent, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium tartrate | 0.9 (M) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | drop | MES | 20 (mM) | pH5.4 |