1M4R
CRYSTAL STRUCTURE OF RECOMBINANT HUMAN INTERLEUKIN-22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.430, 61.610, 73.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.700 * - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 19.100 * |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.700 * | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.350 |
| Total number of observations | 61846 * | |
| Number of reflections | 16382 * | |
| <I/σ(I)> | 14.5 | 3.8 |
| Completeness [%] | 92.7 * | 82.3 |
| Redundancy | 3.8 * | 3.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 300 | 0.9 sodium tartrate, TRITON X-100 detergent, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium tartrate | 0.9 (M) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | MES | 20 (mM) | pH5.4 |
