1M3X
Photosynthetic Reaction Center From Rhodobacter Sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 141.800, 141.800, 187.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.550 |
| Rwork | 0.185 |
| R-free | 0.20500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qov |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.630 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.126 | 0.406 |
| Total number of observations | 564825 * | |
| Number of reflections | 69691 * | |
| <I/σ(I)> | 4.3 | 1.5 |
| Completeness [%] | 97.2 | 92.5 |
| Redundancy | 4.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | POTASIUM PHOSPHATE, LDAO, HEPTANE TRIOL, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | heptano-1,2,3-triol | 3.5 (%) | |
| 2 | 1 | drop | lauryl dimethylamine oxide | 0.1 (%) | |
| 3 | 1 | drop | dioxane | 2.0 (%) | |
| 4 | 1 | drop | potassium phosphate | 0.75 (M) | pH7.4 |
| 5 | 1 | drop | protein | 0.12 (mM) | |
| 6 | 1 | reservoir | potassium phosphate | 1.6 (M) | pH7.4 |
