1M3X

Photosynthetic Reaction Center From Rhodobacter Sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 31 2 1
Unit cell lengths	141.800, 141.800, 187.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.550
Rwork	0.185
R-free	0.20500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qov
RMSD bond length	0.010 *
RMSD bond angle	1.630 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.126	0.406
Total number of observations	564825 *
Number of reflections	69691 *
<I/σ(I)>	4.3	1.5
Completeness [%]	97.2	92.5
Redundancy	4.2	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	POTASIUM PHOSPHATE, LDAO, HEPTANE TRIOL, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	heptano-1,2,3-triol	3.5 (%)
2	1	drop	lauryl dimethylamine oxide	0.1 (%)
3	1	drop	dioxane	2.0 (%)
4	1	drop	potassium phosphate	0.75 (M)	pH7.4
5	1	drop	protein	0.12 (mM)
6	1	reservoir	potassium phosphate	1.6 (M)	pH7.4