1M3H
Crystal Structure of Hogg1 D268E Mutant with Product Oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9500 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.345, 92.345, 211.345 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.660 - 2.050 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ebm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.612 |
| Number of reflections | 33658 | |
| <I/σ(I)> | 23.1 | 2.1 |
| Completeness [%] | 88.4 | |
| Redundancy | 5.2 | 4.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 8000, cacodylate, Calcium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | cacodylate | ||
| 3 | 1 | 1 | Calcium Acetate |
