1M2V

Crystal Structure of the yeast Sec23/24 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.050
Spacegroup name	P 21 21 21
Unit cell lengths	90.309, 126.371, 180.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.750
R-factor	0.21
Rwork	0.210
R-free	0.25300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Sec23 from PDB ENTRY 1M2O
RMSD bond length	0.007
RMSD bond angle	1.420 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.920
High resolution limit [Å]	2.750	2.750
Rmerge	0.062	0.252 *
Total number of observations	190671 *
Number of reflections	49463
Completeness [%]	91.2	63.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	22 *	1.0 M ammonium formate, 18 mM dithiothreitol, 10 % (w/v) xylitol, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP at 273K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35 (mg/ml)
2	1	drop	xylitol	15 (%(w/v))
3	1	drop	ammonium formate	1.0 (M)
4	1	drop	dithiothreitol	18 (mM)
5	1	drop	HEPES	0.1 (M)	pH7.5
6	1	reservoir	ammonium formate	1.9 (M)
7	1	reservoir	HEPES	0.1 (M)	pH7.5