1M2O

Crystal Structure of the Sec23-Sar1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-05
Detector	KODAK
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	47.196, 151.158, 271.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.850 - 2.500
R-factor	0.238
Rwork	0.238
R-free	0.29500 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.470 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.046	0.237 *
Total number of observations	289977 *
Number of reflections	63872
Completeness [%]	92.9	79.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	22 *	12% (w/v) PEG 1500, 10% (w/v) isopropanol, 0.2 M ammonium acetate, 50 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 270K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	44 (mg/ml)
2	1	reservoir	PEG1500	12 (%(w/v))
3	1	reservoir	isopropanol	10 (%(v/v))
4	1	reservoir	ammonium acetate	0.2 (M)
5	1	reservoir	MES	50 (mM)	pH6.5