1M2J

Sir2 homologue H80N mutant-ADP ribose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	90
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	35.682, 85.552, 44.798
Unit cell angles	90.00, 111.99, 90.00

Refinement procedure

Resolution	40.000 * - 1.800 *
R-factor	0.182
Rwork	0.182
R-free	0.21670 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.190 *
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.032 *
Total number of observations	60264 *
Number of reflections	21671 *
Completeness [%]	93.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	18 *	PEG 4000, Sodium Citrate, Isopropanol, pH 6.0, EVAPORATION, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mM)
2	1	reservoir	sodium citrate	100 (mM)	pH6.0
3	1	reservoir	PEG4000	14 (%)
4	1	reservoir	isopropanol	20 (%)