1M24
Trichotoxin_A50E, An Ion Channel-Forming Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS |
| Synchrotron site | SRS |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2001-04-24 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 9.490, 16.850, 31.680 |
| Unit cell angles | 95.77, 98.07, 99.45 |
Refinement procedure
| Resolution | 10.000 - 0.900 |
| R-factor | 0.0762 * |
| R-free | 0.09200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL OF HELICAL PEPTIDE RESIDUES 1-13. |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.026 |
| Data reduction software | SMART (V. 5.054 (BRUKER)) |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 0.950 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.051 | 0.261 |
| Number of reflections | 13427 | |
| <I/σ(I)> | 15.03 | 2.36 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 2.79 | 2.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4 * | METHANOL:ACETONITRILE 1:10, EVAPORATION, RECRYSTALLIZATION, TEMPERATURE 268K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | peptide | 29.6 (mg/ml) |
