1M1S

Structure of WR4, a C.elegans MSP family member

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-28
Wavelength(s)	0.95373, 0.97829, 0.97812,
Spacegroup name	P 21 21 21
Unit cell lengths	25.420, 34.020, 101.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.250 - 1.800
R-factor	0.278
Rwork	0.278
R-free	0.29270
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.250
High resolution limit [Å]	1.800
Number of reflections	8645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	PEG 1500 MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K