1M1H
Crystal structure of Aquifex aeolicus N-utilization substance G (NusG), Space group I222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 81.100, 86.200, 108.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.25900 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.074 * | 0.504 * |
| Number of reflections | 27602 | |
| <I/σ(I)> | 30 | 2.3 |
| Completeness [%] | 98.5 | 98.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 * | 18 * | PEG 4000, 2-Propanol, Sodium Chloride, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES/Tris | 0.1 (M) | pH5.8 |
| 2 | 1 | reservoir | PEG4000 | 10 (%) | |
| 3 | 1 | reservoir | 2-propanol | 20 (%) | |
| 4 | 1 | reservoir | 0.1 (M) | ||
| 5 | 1 | drop | Tris-HCl | 20 (mM) | pH7.0 |
| 6 | 1 | drop | 50 (mM) | ||
| 7 | 1 | drop | DNA | 1 (M) |
