1M1C

Structure of the L-A virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9648
Spacegroup name	P 1 21 1
Unit cell lengths	407.000, 403.200, 572.000
Unit cell angles	90.00, 90.46, 90.00

Refinement procedure

Resolution	8.000 * - 3.400 *
Rwork	0.266
R-free	0.32400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014 *
RMSD bond angle	1.627 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.500 *	3.560
High resolution limit [Å]	3.400 *	3.400 *
Rmerge	0.104	0.433 *
Total number of observations	5151654 *
Number of reflections	2105482 *
<I/σ(I)>	8.3	1.5
Completeness [%]	83.8 *	70.8 *
Redundancy	2.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	22 *	Naitow, H., (2001) J. Struct. Biol., 135, 1. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	3.0-6.0 (%)
2	1	reservoir		1.0 (M)
3	1	reservoir	HEPES	0.1 (M)	pH7.0
4	1	drop	virus	4-56 (mg/ml)