1M18
LIGAND BINDING ALTERS THE STRUCTURE AND DYNAMICS OF NUCLEOSOMAL DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.839, 109.628, 183.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.450 |
| Rwork | 0.222 |
| R-free | 0.25800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.107 | 0.247 |
| Number of reflections | 79809 * | |
| <I/σ(I)> | 4.2 | 2.3 |
| Completeness [%] | 98.4 * | 94.2 |
| Redundancy | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 292 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 40-45 (mM) | ||
| 2 | 1 | reservoir | 35-38.8 (mM) | ||
| 3 | 1 | drop | potassium cacodylate | 20 (mM) | pH6.0 |
| 4 | 1 | drop | protein | 4 (mg/ml) |
