1M0N
Structure of Dialkylglycine Decarboxylase Complexed with 1-Aminocyclopentanephosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 151.290, 151.290, 84.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1dka |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.940 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.000 |
| Rmerge | 0.077 | 0.318 |
| Number of reflections | 28332 | |
| <I/σ(I)> | 18.9 | 11.3 |
| Completeness [%] | 96.1 | 98.1 |
| Redundancy | 3.21 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 15% PEG 4000, 0.2-0.4 M SODIUM PYRUVATE, 0.015 M MES-KOH (PH 6.4) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 5 (mM) | pH7.5 |
| 3 | 1 | drop | PLP | 0.020 (mM) | |
| 4 | 1 | drop | sodium azide | 0.2 (%(w/v)) | |
| 5 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 6 | 1 | reservoir | MES- KOH | 15 (mM) | pH6.4 |
| 7 | 1 | reservoir | sodium pyruvate | 75-200 (mM) | |
| 8 | 1 | reservoir | PLP | 0.020 (mM) |
