1M08

Crystal structure of the unbound nuclease domain of ColE7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2001-08-08
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	37.700, 39.200, 53.200
Unit cell angles	96.80, 103.90, 91.40

Refinement procedure

Resolution	50.000 * - 2.100
R-factor	0.187
Rwork	0.187
R-free	0.24300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cei
RMSD bond length	0.017 *
RMSD bond angle	1.655 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.044 *	0.173 *
Total number of observations	53594 *
Number of reflections	16335
<I/σ(I)>	22.5	7
Completeness [%]	95.0	92.7
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	sodium phosphate, Sodium Chloride, zinc chloride, ammonium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	nuclease ColE7	10 (mg/ml)
2	1	drop		5 (mM)
3	1	drop	ammonium acetate	0.35 (M)
4	1	drop	PEG4000	6.35 (%(w/v))
5	1	drop	Tris-HCl	0.025 (M)	pH7.5
6	1	reservoir		10 (mM)
7	1	reservoir	ammonium acetate	0.7 (M)
8	1	reservoir	PEG4000	12.7 (%)