1M06
Structural Studies of Bacteriophage alpha3 Assembly, X-Ray Crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 290.258, 332.115, 337.698 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 82.450 - 3.500 |
| Rwork | 0.232 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Phix174 virion |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.900 * |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.080 | 0.115 |
| Number of reflections | 597062 | |
| <I/σ(I)> | 20 | 18 |
| Completeness [%] | 75.0 * | 55 * |
| Redundancy | 4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 45 * | 3-5% PEG8000, 100 mM sodium citrate pH 5.0, 1 mM EDTA, 300 mM NaCl, 0.1 mM-mercapto-ethanol, and 0.02 % sodium azide, VAPOR DIFFUSION, SITTING DROP, temperature 318.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 10 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 0.1 (mM) | |
| 5 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 6 | 1 | reservoir | PEG8000 | 3-5 (%) | |
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.0 |
| 8 | 1 | reservoir | glycerol | 40 (%(v/v)) | |
| 9 | 1 | reservoir | sodium azide | 0.02 (%) |
