1LZS

STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	46.990, 162.250, 32.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.17
RMSD bond length	0.014
RMSD bond angle	0.035
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.053 *
Total number of observations	63078 *
Number of reflections	25249 *
Completeness [%]	74.5 *	45.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20.0 (mg/ml)
2	1	drop		1.8 (M)
3	1	drop	sodium phosphate	30 (mM)
4	1	drop		8.0 (mg/ml)
5	1	reservoir		2.0 (M)
6	1	reservoir	sodium phosphate	30 (mM)