1LZR

STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	56.510, 60.910, 34.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.500
R-factor	0.14
RMSD bond length	0.016
RMSD bond angle	0.034
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.500 *	1.500 *
Rmerge	0.029 *
Total number of observations	64767 *
Number of reflections	17996 *
Completeness [%]	92.3 *	87.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *	13 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		1.4 (mg/ml)
3	1	drop	sodium phosphate	30 (mM)
4	1	drop		2.5 (M)